Article · Volume 2, Issue 8 · pp. 220–221
In vitro and In vivo inhibitory effects of Piper longum fruit extracts on mouse Ehrlich ascites carcinoma
K.K. Hullatti1, Uma.D. Murthy2, Shrinath B.R.3
- 1Department of Pharmacognosy and Phytochemistry, National college of Pharmacy, Shimoga - 577 201, India.
- 2Department of pharmacognosy, Govt. College of Pharmacy Bangalore-560 027, India.
- 3Principal Research Scientist, Central Animal Facility, Indian Institute of Sciences, Bangalore. India, India.
Abstract
In order to screen the In vitro and In vivo inhibitory effects of Piper longum fruit extracts on mouse Ehrlich ascites carcinoma., Crude extracts of Piper longum fruits, (petroleum ether (60°–80°), chloroform, ethanol 95%, and water extract) were tested for short term in vitro cytotoxic activity using Ehrlich ascites carcinoma cells, and In vivo cytotoxicity using the Swiss albino mice intraperitoneally inoculated with Ehrlich ascites carcinoma cells. Pet ether and chloroform extracts have shown the significant cytotoxic action on the EAC cell lines. Petroleum ether extract increased the life span of tumor bearing animals by 44.44%. Chloroform extract at a dose of 200 mg/kg body weight increased the life span of tumor bearing animals by 33.33%. Combination therapy of both the extracts increased the life span by 87.5%. These results clearly indicate the further detailed work on these extracts can provide us with an effective non-toxic anti tumor agents.
Keywords: Piper longum; short-term in vitro cytotoxicity; Ehrlich ascites carcinoma
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