Article · Volume 20, Issue 4 · pp. 1359–1366
Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells
Junli Wang1, Rui Wang2, Yaming Du2, Xinzi Xu2, Xin Liu1, Wei Shao1, Guohua Chen1,2
- 1College of Clinical Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei, China
- 2Department of Neurology, Wuhan No. 1 Hospital, Wuhan, Hubei, China
Abstract
Background: Huanglian Jiedu decoction (HLJDD) is a classical herbal formula for treating inflammatory diseases with Traditional Chinese Medicine (TCM). However, the impacts of HLJDD on neuroinflammation and the precise mechanisms involved remain poorly understood.Purpose: In the present study, HLJDD was found to inhibit lipopolysaccharide (LPS) induced neuroinflammatory responses in BV2 microglia via regulating NLRP3 inflammasome activation.Materials and Methods: Anti-inflammatory activity was evaluated with ELISA assay and the polarization changes were observed using immunofluorescent staining. The levels of NLRP3 inflammasome activation were determined using qPCR and Western blotting.Results: The administration of HLJDD successfully countered LPS-induced neuroinflammatory responses by attenuating microglial activation and fostering a phenotypic conversion from the pro-inflammatory M1 to the anti-inflammatory M2. Additionally, HLJDD treatment demonstrated inhibition of NLRP3 inflammasome activation.Conclusion: The findings of the present study may provide insights into how HLJDD could be a therapeutic drug for neuroinflammatory diseases associated with regulating NLRP3 inflammasome and microglial activation.
Keywords: Huanglian Jiedu decoction; microglia; BV2 cell; NLRP3 inflammasome; neuroinflammation
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