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PM · Lens · Inhibitory Effect of Cayratia albifolia C. L. Li Extract on LPS-induced Inflammation of RAW264.7 Cells by Regulating the Nrf2/ HO-1 Axis

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Article · Volume 21, Issue 1 · pp. 207–217

Inhibitory Effect of Cayratia albifolia C. L. Li Extract on LPS-induced Inflammation of RAW264.7 Cells by Regulating the Nrf2/ HO-1 Axis

Yi Tan1, Liang Cao2, Dan Zhou3, Biao Qin3, Yanbin Zhang3, Jianxin Liu4, Kai He4, Bin Li5, Zaiqi Zhang2

  1. 1Department of Clinical Laboratory Medicine, The First Affiliated Hospital, University of South China, Hengyang, Hunan, CHINA.
  2. 2Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua, Hunan, CHINA.
  3. 3School of Clinical Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, CHINA.
  4. 4School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua, Hunan, CHINA.
  5. 5TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.

Abstract

Background: Cayratia albifolia C. L. Li (Jiao Mei Gu (JMG)) has potential therapeutic effects in inflammatory diseases.Objectives: The primary goal of the research was to focus on the antioxidant and anti-inflammatory qualities of C. albifolia and the underlying mechanisms that contribute to these benefits.Materials and Methods: To explore the anti-inflammatory abilities of JMG, we employed enzyme-linked immunosorbent assay and nitric oxide (NO) assay in a lipopolysaccharide (LPS)-induced macrophage inflammation model. Furthermore, flow cytometry was employed to assess the antioxidant effects of C. albifolia, and Western blotting was used to reveal the underlying mechanisms.Results: Ethyl acetate extract of C. albifolia effectively reduced the release of NO, interleukin-6, tumor necrosis factor-α, and prostaglandin E2 in macrophages. Additionally, it prevented the expression of reactive oxygen species. Mechanistically, the extract may downregulate the expression of inducible nitric oxide synthase, cyclooxygenase-2, the nuclear factor-erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) protein expression.Conclusion: The ethyl acetate extract of C. albifolia demonstrated remarkable anti-inflammatory and antioxidant effects by modulating the Nrf2/HO-1 axis.

Keywords: Dong medicine; Cayratia albifolia C. L. Li; inflammation; macrophage; lipopolysaccharide; antioxidant

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