The Extract of Camellia japonica L. Protects against Mice Cerebral Ischemia/Reperfusion Injury via Promoting the H2S-BKCa Pathway

Abstract

Background and Objectives: The effect and mechanism of the extract of Camellia japonica L. (ECJ) on brain injury following cerebral ischemia/reperfusion (I/R) were demonstrated in the present study.Materials and Methods: We detected mice’s brain damage after cerebral I/R and tested neuronal injury following oxygenglucose deprivation/re-oxygenation (OGD/R) to evaluate the neuroprotection of ECJ. Besides, we tested the expressions of hydrogen sulfide (H2S) synthase cystathionine-β-synthase (CBS) and α subunit of large-conductance Ca2+-activated K+ channels (BKα) both in brain tissues and culture neurons. Importantly, the roles of iberiotoxin (IbTX), BKCa channel inhibitor, and CBS inhibitor aminooxyacetic acid (AOAA) on ECJ-mediated neuroprotection were assessed to explore the neuroprotective mechanism of ECJ.Results: ECJ treatment could alleviate the mice’s brain injury following cerebral I/R and reduce the neuronal damage caused by OGD/R in vitro, which was inhibited by IbTX and AOAA. In addition, ECJ could increase the expression of CBS and the α subunit of BKCa channel (BKα) in mice brain tissues and the culture neurons, as well as improve the H2S production. Furthermore, exogenous H2S donor NaHS also improved the BKα expression in OGD/R neurons. Importantly, NaHS alleviated the neuronal injury in vitro, which was inhibited by IbTX as well.Conclusion: ECJ can protect against mouse cerebral I/R injury, the mechanism of which is correlated with promoting the CBS/H2S-BKCa pathway.

Keywords: Extract of Camellia japonica L; brain injury; hydrogen sulfide; iberiotoxin; aminooxyacetic acid