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PM · Lens · New Polysaccharides from Hygrophorus olivaceoalbus (HO-P) and Agrocybe aegerita (AA-P) and their Biological Activities

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Article · Volume 21, Issue 2 · pp. 507–520

New Polysaccharides from Hygrophorus olivaceoalbus (HO-P) and Agrocybe aegerita (AA-P) and their Biological Activities

Tong Yang1,2, Hui Zhao3, Xiang Ding1,2,3,4, Yiling Hou1

  1. 1Organizational Repair Material Engineering Technology Collaborative Innovation Center of Sichuan Province, College of Life Sciences, China West Normal University, Nanchong, Sichuan Province, China.
  2. 2College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan Province, China.
  3. 3Academy of Agricultural Sciences of Dazhou City, Dazhou, Sichuan Province, China.
  4. 4Xichong Xinghe Biotechnology Co., Ltd, Xichong, Sichuan Province, China.

Abstract

Background: Fungal polysaccharides are an essential active polymer compound in human cells. A clear understanding of the structure of polysaccharides is the foundation for conducting research and utilization of polysaccharides.Objectives: To explore the structure and immunomodulatory activities of polysaccharides from Hygrophorus olivaceoalbus and Agrocybe aegerita.Materials and Methods: High-performance liquid chromatography, high-performance gel-permeation chromatography, nuclear magnetic resonance, and gas chromatography–mass spectrometry analysis were utilized to analyze the structure of Hygrophorus olivaceoalbus polysaccharide (HO-P) and Agrocybe aegerita polysaccharide (AA-P). The immunomodulatory activities of these polysaccharides were also studied.Results: The HO-P consisted of (1→6)-Galp, (1→4)-Glup, (→1)-Glup, and (1→4,6)-D-Manp with the ratio of mannose, galactose, and glucose being 1:1:2. The AA-P was consisted of (1→4,6)-Glup, (1→4)-Arap, and (1→6)-Galp with the ratio of galactose, glucose, and arabinose being 3:2:1. AA-P and HO-P could both mainly by reducing G0/G1 phase, which leads to the significant proliferation of B cells and RAW264.7 cells, respectively. At the same time, AA-P and HO-P could significantly stimulate the necrosis factor-α secretion of T cells, IgA, IgD, IgE, IgG, and IgM secretion of B cells and tumor necrosis factor-α secretion of RAW264.7 cells, but neither of them could stimulate interleukin-1→ secretion of RAW264.7 cells.Conclusion: Due to its better branching degree, AA-P is more conducive to forming a triple-helix, three-dimensional configuration, as well as better solubility and smaller molecular weight, thus exhibiting higher biological immune activity against immune cells than HO-P.

Keywords: Hygrophorus olivaceoalbus; Agrocybe aegerita; polysaccharide; structure characterization; biological activities

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