GLA-loaded Liposome Albumin Particles-mediated p53 Targets Cervical Cancer Cell Transplant Tumors

Abstract

Background: p53 is a phosphorylated glycoprotein whose expression is abnormally elevated in various human tumors. p53 also regulates a variety of tumor cells, but research on cervical cancer has not yet been reported.Objectives: This study intends to investigate the role of targeting p53 in cervical cancer.Materials and Methods: Electron microscopy was used to assess the size of protein particles, 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-2H-tetrazolium bromide (MTT) was used to detect the OD value of nanocomposites at different concentrations using a UV spectrophotometer, MTT and cell cloning were used to detect cell proliferation expression ability, and reverse transcriptase polymerase chain reaction (RT-PCR) to detect p53 expression. Immunofluorescence measured nuclear factor kappa B (NF-κB) expression in cells, and western blotting assessed the relative expression of p53 and glyceraldehyde-3- phosphate dehydrogenase (GAPDH) proteins. The detection mechanism was further explored through in vivo studies.Results: Glutaraldehyde (GLA)-loaded liposomal albumin particles showed a positive zeta value, indicating that the CS coating process of GLA was successful. Our data show that the experimental group showed a concentration-dose-dependent growth relationship, indicating that GLA-loaded liposome albumin particles have better antitumor activity in cervical cancer cell lines. Compared with most GLA-loaded liposomal albumin particle groups, p53 expression was inhibited, and the expression of p53 was downregulated in GLA-loaded liposomal albumin particle cervical cancer. In vivo results showed that the measured tumor volume decreased after treatment with GLA-loaded liposomal albumin particles, and mice treated with GLA-loaded liposomal albumin particles had longer survival times. Induction of NF-κB in tumors may be a powerful strategy allowing GLA-loaded liposomal albumin particles to avoid apoptosis and possibly after cytotoxic chemotherapy.Conclusion: This study demonstrates that GLA-loaded liposomal albumin particles may participate in the development and progression of human cervical cancer cells by targeted regulation of NF-κB activity.

Keywords: Glutaraldehyde; liposomal albumin particles; cervical cancer; p53