A Study on the Effect of Ganoderic Acid A on Nociceptive and Inflammatory Mice Models

Abstract

Background: Inflammatory illnesses and severe tissue injuries are frequently accompanied by pain. A widespread global health issue that has a significant negative impact on people’s quality of life everywhere is inflammation and the pain it causes.Objectives: In this study, we sought to evaluate Ganoderic acid A’s antinociceptive and anti-inflammatory properties in mice models.Materials and Methods: The current study aims to investigate the influence of Ganoderic acid A on animal models of inflammation and nociceptive response produced by heat and chemicals. Ganoderic acid A’s antinociceptive activity was examined using acetic acid-induced writhing, formalin and capsaicin-induced nociception, the tail immersion test, and the hot plate method. Ganoderic acid A’s anti-inflammatory effects were investigated utilizing carrageenan-induced inflammation, as well as the assessment of pro-inflammatory cytokine levels in mice. Additionally, the open-field test was used to examine behavioral changes in the mice.Results: The findings of this work unequivocally showed that Ganoderic acid A significantly reduced the various thermallyand chemically-induced nociception in mice. Ganoderic acid A considerably increased the delay time in the hot plate and tail immersion tests. Capsaicin and formalin-stimulated models showed better licking times, and acetic acid-induced nociception was considerably reduced. Ganoderic acid A also effectively suppressed carrageenan-induced inflammation and decreased the synthesis of pro-inflammatory regulators including tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in the experimental animals. However, it demonstrated only minor improvements in the behavioral assessment.Conclusion: Thus, these findings, when paired with future investigations, will provide a better knowledge of Ganoderic acid A’s antinociceptive and anti-inflammatory capabilities, allowing for the development of a novel analgesic drug. To corroborate the preclinical findings, however, rigorously planned clinical investigations are desperately needed.

Keywords: Antinociceptive; anti-inflammatory; Ganoderic acid A; carrageenan; pain