Poncirin Inhibits Cell Viability and Induces Caspase-mediated Apoptosis in Cervical Cancer Cells

Background: Cervical cancer continues to pose a considerable global health concern, disproportionately impacting women in low- and middle-income nations due to various barriers. Although mostly preventable with immunization and screening, it ranks as the fourth most widespread cancer among women worldwide. Objectives: The present study examines the capacity of poncirin to suppress viability and promote apoptosis in cervical cancer HeLa cells. Materials and Methods: In this work, a 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) test was utilized to study the effect of poncirin (5–160 μM) on the growth of HeLa cells. Apoptosis in the experimental HeLa cells was examined using a dual staining technique. The activities of caspase enzymes in the cells were evaluated using kits. Results: The MTT assay findings indicated a substantial decrease in HeLa cell growth after treatment with poncirin. The findings of the dual staining test confirmed the initiation of apoptosis in the poncirin-treated HeLa cells. Moreover, the poncirin treatment significantly elevated caspase enzyme activities in the HeLa cells, thereby facilitating apoptotic induction. Conclusion: The present findings indicate that poncirin treatment significantly inhibits growth and enhances caspase-mediated apoptotic cell death in HeLa cells. Consequently, it can serve as an anti-tumor candidate for the treatment of cervical cancer.