Inhibition of Oral Carcinoma Cell Proliferation by miR-15b Carrying Pterostilbene and Related Mechanisms

Background: MicroRNA-15b (miR-15b) plays a cancer-suppressing role in various tumors, while sandalwood astragalus, as a natural antioxidant, has significant anti-cancer potential. However, the synergistic mechanism of miR-15b and Rosewood stilbene in oral cancer remains unclear. Objectives: This study investigated the effects of miR-15b combined with Rosewood astragalus treatment on oral cancer KB cells. Materials and Methods: Oral cancer KB cells were divided into the miR-15b mimics group, miR-15b inhibitor group, and negative control group, followed by measuring cell activities and B-cell lymphoma 2 (Bcl-2) expression. Results: Transfection of miR-15b carrying pterostilbene mimic promoted overexpression of KB cell lines, inhibiting KB cell proliferation and increasing caspase-3 activity (p < .05). Overexpression of miR-15b carrying pterostilbene inhibited Bcl-2 expression (p < .05). However, transfection inhibitors significantly inhibited the expression of miR-15b carrying pterostilbene, promoting cell proliferation, upregulating Bcl-2, and inhibiting caspase-3. However, the changes in the expression of pterostilbene carried by miR-15b did not alter cell invasion (p > .05). Conclusion: Pterostilbene carried by miR-15b downregulates Bcl-2 expression, increasing caspase-3 activity, promoting tumor cell apoptosis, and inhibiting the proliferation of oral cancer cells.