Effect of Baicalein Inhibiting miR-155 through STAT3 Signaling Pathway on Inflammatory Response in Mice with Acute Appendicitis

Background: Acute appendicitis is caused by infection and inflammation of the appendix. Baicalein (ABC) can reduce inflammation through various mechanisms, but its water solubility is poor. Liposomes have good biocompatibility and drug delivery ability. Objectives: This study aims to explore the therapeutic effect of baicalein liposome nanoparticles (LNS-ABC) with a good targeting effect on acute appendicitis and provide a basis for developing new drugs. Materials and Methods: The LNS-ABC complex was prepared, and a mouse model of acute appendicitis was constructed. The levels of inflammatory factors in mice were measured. The relationship between miR-155 and signal transducer and activator of transcription 3 (STAT3) was investigated by luciferase assay. Using miR-155 agonists and inhibitors and STAT3 agonists and inhibitors, the mechanism of action of LNS-ABC in mice with acute appendicitis was investigated. Results: ABC can effectively inhibit the inflammatory response in acute appendicitis, and the inhibitory effect of LNS-ABC is more effective. Moreover, LNS-ABC inhibits the expression of miR-155 by regulating and inhibiting the STAT3 signaling pathway, thereby improving inflammation in acute appendicitis. Conclusion: Baicalein can effectively inhibit the inflammatory response of acute appendicitis mice, and LNS-ABC has a better curative effect. LNS-ABC can inhibit the expression of miR-155 by inhibiting the STAT3 signaling pathway, thus improving the inflammatory response of acute appendicitis. LNS-ABC is an essential strategy for the treatment of acute appendicitis and its inflammatory reaction.