Effects of Resveratrol on Cardiomyocyte Hypoxia-reoxygenation Injury Induced by High Glucose and Its Relationship with NRG1/ErbB Pathway

Background: Research has found that diabetes and cardiomyopathy are partially caused by hypoxia-reoxygenation damage to myocardial cells in a high-glucose environment. Resveratrol (RSV) exhibits protective effects on the cardiovascular system, and the neuregulin-1/erythroblastic leukemia viral oncogene homolog (NRG1/ErbB) pathway plays a crucial role in cardiac development and functional regulation. Objectives: This study aims to explore the effect of RSV on myocardial cell hypoxia-reoxygenation injury caused by high glucose (HG) and its relationship with NRG1/ErbB. The relationship between the ErbB pathway can provide new ideas for preventing and treating diabetic cardiomyopathy. Materials and Methods: The experimental mice were randomly divided into the negative control (NG) group, the HG group, the HG+H/R group, the 0.5 RSV group, the 1.0 RSV group, the 1.5 RSV group, the 1.5 RSV+NRG1 group, and the 1.5 RSV+NRG1+AG1478 group by a blind selection method. Cell proliferation, invasion, and expression of caspase-3, ErbB4, PI3K, Akt, MAPK, and reactive oxygen species (ROS) were detected. Results: RSV can effectively reduce the apoptosis of myocardial injury caused by HG and can improve its activity; specific blocking of the NRG1 signaling pathway can reverse the protective effect of RSV on myocardial injury caused by HG to a certain extent, and activating NRG1 pathways can amplify their protective effects. Conclusion: RSV can reduce the expression of caspase-3 through the NRG1/ErbB signaling pathway, not only reducing cell apoptosis but also reducing the hypoxia-reoxygenation injury of cardiomyocytes caused by HG to a certain extent, further playing a protective role in cardiomyocytes.