Albiflorin Inhibits Cell Viability and Promotes Apoptosis in Breast Cancer MDA-MB-231 Cells via Regulating Bcl-2 Family Proteins Rui Gao1*

Background: Breast cancer remains the most widely diagnosed cancer among women worldwide and a major cause of cancer-associated deaths, posing a substantial and escalating burden on public health systems worldwide. Objectives: This study was intended to evaluate the anti-cancer effects of albiflorin against breast cancer cells. Materials and Methods: The effect of albiflorin treatment at different dosages (1–50 µM) on the growth of MDA-MB-231 cells was evaluated using the MTT assay. The mitochondrial membrane potential (MMP) level and apoptosis in the albiflorintreated breast cancer cells were assessed using fluorescent staining assays. The apoptotic proteins Bax, Bid, and Bcl-2 levels in the albiflorin-treated breast cancer cells were studied using kits. Results: The findings of the MTT test revealed that albiflorin treatment significantly diminished the MDA-MB-231 cell viability. The albiflorin treatment diminished the MMP levels and triggered apoptosis in the breast cancer cells, as shown by fluorescent staining studies. Additionally, the albiflorin-treated MDA-MB-231 cells showed augmented pro-apoptotic Bax and Bid protein levels and reduced anti-apoptotic Bcl-2 levels. Conclusion: The present study suggests that albiflorin can inhibit viability and promote apoptosis in breast cancer cells. Thus, the current data suggest albiflorin as a promising candidate that can be utilized for breast cancer management in the future.