Development of a simple and sensitive spectrophotometric method for the simultaneous determination of asiaticoside and wedelolactone in a polyherbal formulation.

Abstract

A simple, sensitive spectroscopic method was developed for the simultaneous determination of asiaticoside and wedelolactone in mixture of Centella asiatica extract and Eclipta alba extract as well as in a polyherbal formulation containing these extracts. Asiaticoside has an absorption maxima at 278nm, the molecule obeys Beer’s law in the concentration range 10-100 µg/mL, while wedelolactone has the absorption maxima at 351nm and the linearity range is 1-10 µg/mL. The method for simultaneous estimation was based on the property of additive values of absorbance. The proposed method is reproducible and statistically validated. The average % recovery for asiaticoside is 98.63 % and for wedelolactone is 98.41 %. The developed method is an effective approach for simultaneous quantitative determination, which could be used for quality control of polyherbal preparations containing extracts of Centella asiatica and Eclipta alba.

Keywords: Asiaticoside; Polyherbal; Simultaneous Determination; Wedelolactone