Article · Volume 3, Issue 9 · pp. 52–53

Rapid Micropropagation of Tylophora indica

R.K. Nema¹, K.G. Ramawat², G.D. Gupta³, Y.S. Tanwar¹, Meeta Mathur²

¹Bhupal Nobles’ College of Pharmacy, Udaipur, India.
²Laboratory of Biomolecular Technology, College of Science, MLS University, Udaipur, India.
³Sagar Institute of Pharmacy, Sagar, India.

Abstract

In vitro raised shoot culture of T. indica was established on MS medium with 2.5 mg/l BA and 0.1mg/l IAA. An excellent rate of shoot multiplication was achieved. The tissue culture-generated shoots were rooted in the medium containing IAA for in vitro development of roots. A highly regenerative system has been developed by using leaf explants from mature plant. This system can be effectively used to multiply the plant material.

Keywords: Micropropagation; Tylophora asthmatica; Tylophora indica

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