Article · Volume 4, Issue 16 · pp. 269–270
Antiproliferative effects of a flavonoid and saponins from Astragalus hamosus against human tumor cell lines
I. Krasteva1, G. Momekov2, P. Zdraveva1, S. Konstantinov2, S. Nikolov1
- 1Department of Pharmacognosy, Faculty of Pharmacy, 2 Dunav St., 1000 Sofia, Bulgaria.
- 2Lab. of Molecular Pharmacology and Experimental Chemotherapy, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, 2 Dunav St., 1000 Sofia, Bulgaria.
Abstract
The antiproliferative effects of a flavonol glycoside rhamnocitrin 4’-βD-galactopyranoside and mixture of two saponins, obtained from Astragalus hamosus L. (Fabaceae), were tested in a panel of human tumor cell lines, using the MTT-dye reduction assay. The saponin mixture caused concentration-dependent inhibition of malignant cell proliferation, while the flavonoid exerted only marginal effects. These data were corroborated by an ELISA test to assess the characteristic for apoptosis DNA-fragmentation after treatment with the tested compounds. At equipotent concentrations the saponin mixture was found to be superior inducer of apoptosis as compared to the flavonoid.
Keywords: Astragalus hamosus; Fabaceae; flavonoids; saponins; MTT-assay; apoptosis
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