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PM · Lens · Rapid Micropropagation of Curculigo orchioides in Shake Flask Culture

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Article · Volume 4, Issue 16 · pp. 315–316

Rapid Micropropagation of Curculigo orchioides in Shake Flask Culture

R.K. Nema1, K.G. Ramawat2, J.M. Merillon3

  1. 1S.D.College of Pharmacy & Vocational Studies, Muzaffarnagar.
  2. 2Laboratory of Biomolecular Technology, College of Science, MLS University, Udaipur.
  3. 3Faculte’ des Sciences, Pharmaceutiques, Universite’ de Bordeaux II, France.

Abstract

Curculigo orchioides (Hypoxidaceae) is a monocot stemless perennial herb. It is an endangered plant species of medicinal importance. Efficiency of two different culture systems, viz., different gelling agents as static cultures and shake flask cultures were compared for the production of bulbils. A protocol for micropropagation of C. orchioides through direct bulbil formation from leaf explants in shake flask cultures has been standardized. Effect of different concentrations of sucrose and different sugars has also been studied. Plantlets developed in vitro were successfully transferred to soil with a high rate of survivability (90%) and were comparable to natural population in growth and vigour.

Keywords: Curculigo orchioides; Microprppagation; Shake Flask Culture

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