Antioxidant, antimicrobial and cytotoxicity properties of the methanolic extract from Grewia tiliaefolia Vahl

Abstract

The whole, fresh barks of Grewia tiliaefolia Vahl. (Tiliiacea), were extracted with methanol. All obtained extracts were evaluated for their antioxidant, antimicrobial and cytotoxicity properties. Two different methods (disc diffusion and agar dilution methods) were employed to evaluate the antimicrobial activities of plant extracts against B. Sterarothermophilus, B. Aerogeuosa, B. Coagulans, K.Pneumoniae, L. Licjmani, Shigell and P. Cepacia. The minimum inhibitory concentration (MIC) values of Grewia tiliaefolia methanolic extract was found to be 125 µg/ml against B. Sterarothermophilus, 31.25 µg/ml against L. Licjmani and 62.5 µg/ml against P. Cepacia. The antioxidant potential was evaluated using following in vitro methods: Nitric oxide radical inhibition activity (superoxide anion) and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The antioxidant activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (13.65 µg/ml IC50 value) and superoxide anion radicals (44.0 µg/ml IC50 value) and reducing power. Cytotoxicity study was done using Vero and HEp-2 cell line. CTC50 values were found to be 205 ± 13.23 µg/ml and 345 ± 12.65 µg/ml. The results showed that Grewia tiliaefolia extract had great potential as antioxidant, antimicrobial and very good anticancer agent.

Keywords: Minimum inhibitory concentration; Natural antioxidants; Super oxide radical inhibition; DPPH; Antimicrobial activity