Article · Volume 5, Issue 17 · pp. 71–74
A Sensitive Reversed Phase HPLC Method for the Determination of Curcumin
Yadav Vivek Ramshankar1, Sarasija Suresh1
- 1Department of Pharmaceutics, Al-Ameen college of Pharmacy, Hosur Road, Bangalore - 560027, India.
Abstract
A simple, precise, rapid and accurate, binary reverse phase high performance liquid chromatographic method has been developed for the determination of curcumin a natural drugs with short run time. Chromatographic separation was achieved by using Merck C15 (250 cm X 4.6 mm) Column with mobile phase acetonitril: tetrahydrofuran: 2% acetic acid 50:30:20 (2%) was used. The flow rate was 0.7 ml/min. The retention time was 4.587 minutes. The limit of detection and limit of quantification of curcumin were between 3.68 to 8.125 ng/ml for 50-µL injection volumes. The percentage recovery of curcumin was found to be 97.2 to 98.4 and Relative standard deviation was 0.345 % and 1.160 %. The developed HPLC method can therefore be applied to both in vitro studies of curcumin formulations as well as drug estimation in biological samples.
Keywords: Curcumin; HPLC; natural drug; limit of detection
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