Article · Volume 5, Issue 18 · pp. 145–150

In Vitro Propagation of Desmodium gangeticum (L.) DC. from Cotyledonary Nodal Explants

U.R. Vishwakarma¹, A.M Gurav¹, P.C. Sharma¹

¹Regional Research Institute (Ay.), Kothrud, Pune - 4110 38 (MH), India.

Abstract

An in vitro procedure for rapid multiplication of medicinally important plant Desmodium gangeticum (L.) DC. (Fabaceae), has been developed using cotyledonary nodal explant. An average of 9.2 shoots per explant were obtained by culturing cotyledonary nodal explant on Murashige and Skoog’s medium containing 8.8 µM BAP and 21.2 µM NAA, in combination, within 28 days. These shoots were rooted on half strength MS medium supplemented with IAA 17.1 µM. Rooted plantlets were hardened using 1:1:1 mixture of soil, river sand and vermiculite under green house conditions.

Keywords: Desmodium gangeticum; cotyledonary nodes; regeneration; growth regulators

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