Prevention of high-fructose diet induced Insulin resistance by Nyctanthes arbortristis and Calotropis gigantea in rats

Abstract

We have investigated the effect of Nyctanthes arbortristis (50, 100, 200 mg/kg) and Calotropis gigantea leaves and flower chloroform (10, 20, 50 mg/kg) and Calotropis gigantea flower petroleum ether extracts (10, 20, 50 mg/kg) in high-fructose diet induced insulin resistance in rats. The fasting serum glucose, insulin, triglyceride and cholesterol levels were measured in blood serum for 27 days of treatment. The fasting serum glucose, insulin, insulin resistance index (FIRI) levels of high-fructose diet (control) rats significantly (P<0.001 vs. normal) increased, like wise, serum triglyceride, cholesterols significantly (P<0.001-P<0.01 vs. normal) increased. The Nyctanthes arbortristis and Calotropis gigantea leaves and flower treatment prevent significantly (P<0.001-P<0.01) vs. control) increase serum glucose, insulin, levels in high fructose-diet treated rats, except in glucose Calotropis gigantea leaves 50 mg/kg, while significantly (P<0.05-P<0.01 vs. control) decreased in triglyceride, cholesterol, except in triglyceride Nyctanthes arbortristis leaves 50 mg and in cholesterol Nyctanthes arbortristis leaves and flowers 50 mg. Further more, high-fructose diet (control) had higher in FIRI (P<0.001) than normal. In contrast, Nyctanthes arbortristis and Calotropis gigantea significantly (P<0.001) decreased FIRI in the high-fructose diet treated rats.

Keywords: Calotropis gigantea; Cholesterol; FIRI; Insulin resistance; Nyctanthes arbortristis; Triglyceride