Article · Volume 5, Issue 20 · pp. 336–342
Agrobacterium rhizogenes Mediated Genetic transformation of Abrus precatorius L.
Karwasara Vijai Singh1, VK Dixit1
- 1Biotechnology Research Laboratory, Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar-470003 (M.P.) India, India.
Abstract
Abrus precatorius L. known as Indian liquorice is a common deciduous vine containing sweet principle compound known as glycyrrhizin. Hence it can be used as a very good substitute for Liquorice. Genetic transformation has proved to be an effective way to enhance secondary metabolites in plant cell cultures. The transformation of Abrus precatorius L. mediated by Agrobacterium rhizogenes was studied using three bacterial strains i.e. MTCC 532, MTCCC 2364 and NCIM 5140. Putative hairy roots were obtained after the transformation. The effects of bacterial strains, bacterial concentration, acetosyringone and co-cultivation pH on transformation of Abrus were investigated. Co-cultivation with Strain MTCC 532 for 2 days with 100 μmol L −1 acetosyringone at pH 6.5 provided the optimal conditions under which transformation frequency approached 84%.
Keywords: Abrus precatorius; Agrobacterium rhizogenes; Genetic transformation; Hairy roots
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