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PM · Lens · Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures

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Article · Volume 7, Issue 25 · pp.

Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures

Chunpeng Wan2, Yanying Yu3, Shouran Zhou4, Wei Liu2, Shuge Tian5, Shuwen Cao2,3

  1. 1State Key Laboratory of Food Science & Technology, Nanchang University, Nanchang - 330 047, Jiangxi, China.
  2. 2Department of chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China.
  3. 3Jiangxi University of Traditional Chinese Medicine, Nanchang - 330 006, Jiangxi, China.
  4. 4Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, Xinjiang, China.

Abstract

Background: Extraction temperature influences the total phenolic content (TPC), total flavonoid content (TFC) of medicinal plant extracts to a great extend. TPC and TFC are the principle activity constituents present in the plant. The effects of extraction temperature on TPC, TFC and free radical-scavenging capacity of Gynura divaricata leaf extracts are worth to study.Materials and Methods: Folin–Ciocalteu and aluminum chloride colorimetric assay were used to determine the TPC and TFC of Gynura divaricata leaf extracts at different temperatures. The antioxidant and free radical-scavenging activity were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino- bis-(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and phosphomolybdenum methods.Results: TPC and TFC were significantly elevated with increasing extraction temperature (from 40°C to 100°C). However, TPC and TFC were not significantly different (P > 0.05) at the extraction temperatures 90°C and 100°C. Also, the extracts obtained at a higher temperature exhibited a significant free radical-scavenging activity compared with extraction at lower temperatures (P < 0.05). The TPCs (13.95–36.68 mg gallic acid equivalent/g dry material) were highly correlated with DPPH (R2 = 0.9229), ABTS (R2 = 0.9951) free radical-scavenging capacity, and total antioxidant activity (R2 = 0.9872) evaluated by phosphomolybdenum method.Conclusion: The TPC and TFC of G. divaricata leaf was significantly influenced by the extraction temperatures, which were the main antioxidant constituents present in the G. divaricata plant.

Keywords: Antioxidant activity; extraction temperature; Gynura divaricata; total phenolic content; total flavonoid content

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