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PM · Lens · A high-performance molluscicidal ingredient against Oncomelania hupensis produced by a rhizospheric strain from Phytolacca acinosa Roxb.

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Article · Volume 7, Issue 28 · pp. 277–283

A high-performance molluscicidal ingredient against Oncomelania hupensis produced by a rhizospheric strain from Phytolacca acinosa Roxb.

Danzhao Guo2,3, Jun Chen4, Yidan Liu4, Hu Yao4, Fang-An Han4,5, Jing Pan4

  1. 1School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.
  2. 2College of Life Science and Technology, Henan Institute of Science and Technology, Henan, China.
  3. 3School of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, China.
  4. 4Zhenjiang Center for Disease Control and Prevention, Zhenjiang, Jiangsu, People's Republic of, China.

Abstract

Background: Snail (Oncomelania hupensis) control is an important and effective preventive strategy in schistosomiasis control programs, and screening microbial molluscicidal agents is one of the most promising categories in biomolluscicides.Objective: To purify and identify the molluscicidal ingredient (MI) obtained from strain SL-30’s exocellular broth.Materials and Methods: The active extracts extracted from SL-30’s exocellular broth was purified on a silica gel column guided by molluscicidal activity assay against Oncomelania hupensis, then the MI was obtained. NMR spectroscopy and LC-MS/MS analysis was used to identify the molecular structure of the MI.Results: Molluscicidal activity bioassay showed that the MI exhibited significant molluscicidal activity with the LC50 values of 0.101, 0.062, and 0.022 mg/L, respectively, in the case of exposure period of 24 h. From 1H NMR, 13C NMR, 1H-1H COSY, and 1H-13C HSQC spectra, partial important structure fragment was obtained, and the relative molecular weight of the MI showed 326 according to LC-MS analysis. Then, on these grounds, it was indicated that the molecular structure of the MI had a higher similarity to Gliotoxin with the molecular formula of C13H14N2O4S2. The quasi-molecular ion of m/z 325.45 was further analyzed by MS2 as the parent ion, and two daughter ions obtained at m/z 295.11 [M-CH2OH]- and m/z 261.08 [M-CH2OH -2S]−.Conclusion: The MI was finally confirmed as Gliotoxin.

Keywords: Gliotoxin; Liquid chromatography-mass spectrometry / mass spectrometry analysis; molluscicidal ingredient; nuclear magnetic resonance spectroscopy; Oncomelania hupensis; Phytolacca acinosa Roxb; strain SL-30

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