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PM · Lens · Chemical composition and biological evaluation of essential oils of Pulicaria jaubertii

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Article · Volume 9, Issue 33 · pp. 28–32

Chemical composition and biological evaluation of essential oils of Pulicaria jaubertii

Ghada A. Fawzy2,3, Hanan Y. Al Ati2, Ali A. El Gamal2,4

  1. 1Department of Pharmacognosy, College of Pharmacy, King Saud University. P.O. Box 22452, Riyadh 11495, KSA.
  2. 2Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562.
  3. 3Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.

Abstract

Background: The present study reports and compares the results of Gas Chromatographic-Mass analyses of Pulicaria jaubertii leaf (P-1) and root (P-2) essential oils, as well as their in vitro antimicrobial and cytotoxic activities.Materials and Methods: The chemical composition of P-1 and P-2 essential oils of P. jaubertii, was investigated by GC-MS. Moreover, the essential oils were evaluated for their antimicrobial activity using the broth micro-dilution assay for minimum inhibitory concentrations (MIC). The crystal violet staining method (CVS) was used for evaluation of their cytotoxic activity on HEPG-2 and MCF-7 human cell lines.Results: This investigation led to the identification of 16 constituents in P-1, and 23 constituents in P-2, representing 99.92% and 94.74% of the oils respectively. Oxygenated monoterpenes were found to be the major group in both P-1 (99.47%) and P-2 (89.88%). P-1 consists almost entirely of p-Menth-6-en-2-one (Carvotanacetone, 98.59%). P-2 is characterized by high contents of each of Dimethoxydurene (38.48%), Durenol (26.89%) and 2′,4′-Dimethoxy-3′-methylacetophenone (20.52%). Both oils showed moderate antimicrobial activity against the Gram-positive strains and C. albicans. However, no activity was shown against Gram-negative bacteria. P-1 showed a significant cytotoxic activity against both MCF-7 and HEPG-2 (IC50= 3.8 and 5.1 µg/ml, respectively), while P-2 showed selective cytotoxic activity against MCF-7 cell line (IC50 = 9.3 µg/ml).Conclusion: The potent cytotoxic and moderate antimicrobial activities of P-1 may be attributed to its high content of Carvotanacetone.

Keywords: Carvotanacetone; cytotoxicity; essential oil; Pulicaria jaubertii

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