Article · Volume 9, Issue 35 · pp. 196–201
Interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42)
Li Shuai1, Zhi Chen2, Ruixi Gao1, Tianming Yang1
- 1College of Pharmacy, South-Central University for Nationalities, Minyuan Road 708, Hongshan District, Wuhan, Hubei, 430074, China.
- 2Center of Analysis, Guangdong Medical College, Dongguan, 523808, P. R, China.
Abstract
Background: Lobed kudzuvine root and rhizoma chuanxiong are effective drugs in traditional Chinese medicine.Objective: Extracts of the two medicines were investigated for their in vitro of beta-amyloid (Aβ1-42)-aggregation-and acetylcholinesterase (AChE)-inhibitory activities.Materials and Methods: The interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42) were studied by Michaelis–Menten equations, Thioflavin T (ThT) fluorescence analysis and transmission electron microscope (TEM).Results: Inhibition of acetylcholinesterase showed that 1-butanol fraction of the two medicines were noncompetitive inhibition, apparent inhibition constants were 9.947 and 7.1523. ThT fluorescence analysis and TEM results indicated that inhibition of the water fraction and 1-butanol fraction (both lobed kudzuvine root and rhizoma chuanxiong) was better.Conclusion: The result supported further research on chemical constituents and pharmacological mechanisms.
Keywords: Acetylcholinesterase; Beta-amyloid; lobed kudzuvine root; rhizoma chuanxiong
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