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PM · Lens · Cytoprotective and antioxidant effects of phenolic compounds from Haberlea rhodopensis Friv. (Gesneriaceae)

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Article · Volume 9, Issue 36 · pp. 294–301

Cytoprotective and antioxidant effects of phenolic compounds from Haberlea rhodopensis Friv. (Gesneriaceae)

Magdalena Kondeva-Burdina1, Dimitrina Zheleva-Dimitrova2, Paraskev Nedialkov2, Ulrich Girreser1

  1. 1Mitka Mitcheva Departments of Pharmacology, Pharmacotherapy and Toxicology, Germany.
  2. 2Pharmacognosy, Faculty of Pharmacy, Medical University of Sofi a, Dunav 2 Str., Sofi a-1000, Bulgaria, Germany.
  3. 3Department of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Christian-Albrechts-University of Kiel, Gutenbergstr. 76, 24118 Kiel, Germany.

Abstract

Background: Haberlea rhodopensis Friv. (Gesneriaceae) is a rare poikilohydric endemic and preglacial relict growing in Balkan Peninsula. Previous investigations demonstrated strong antioxidant, antimicrobial and antimutagenic potential of alcoholic extract from the plant.Objective: The isolation of known caffeoyl phenylethanoid glucoside – myconoside and fl avone- C-glycosides hispidulin 8-C-(2-O-syringoyl--glucopyranoside), hispidulin 8-C-(6-O-acetyl-2-O- syringoyl--glucopyranoside), and hispidulin 8-C-(6-O-acetyl--glucopyranoside) from the leaves of H. rhodopensis was carried out. The aim of this study was to investigate cyto-protective and antioxidant effects of isolated compounds.Materials and Methods: Antioxidant activity of isolated substances was examined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino- bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals; ferric reducing antioxidant power (FRAP) assay and inhibition of lipid peroxidation (LPO) in linoleic acid system by ferric thyocianate method. The compounds were investigated for their possible protective and antioxidant effects against tert-butyl hydroperoxide-induced oxidative stress in isolated rat hepatocytes. The levels of thiobarbituric acid reactive substances were assayed as an index of LPO. Lactate dehydrogenase leakage, cell viability, and reduced glutathione depletion were used as signs of cytotoxicity.Results: Myconoside demonstrated the highest DPPH radical scavenging, ABTS, FRAP, and antioxidant activity in linoleic acid system as well as the highest and statistically most signifi cant protection and antioxidant activity against the toxic agent.Conclusion: Phenolic compounds isolated from H. rhodopensis demonstrated signifi cant cytoprotective, radical scavenging potential, and inhibit lipid peroxidation, moreover, myconoside was found to be a new powerful natural antioxidant.

Keywords: Antioxidant activity fl avone-C-glycosides; cytoprotection; Haberlea rhodopensis; myconoside

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