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Asian Journal of Biological and Life sciencesNOW IN
Article

Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics

Kirti Rani Sharma1★★ Corresponding author

  1. 1Assistant Professor II, Amity Institute of Biotechnology, Amity University, Sec-125, Gautam Buddha Nagar, Noida-201303 (UP), INDIA.

CORRESPONDENCE

Kirti Rani Sharma

Assistant Professor II, Amity Institute of Biotechnology, Amity University, Sec-125, Gautam Buddha Nagar, Noida-201303 (UP), INDIA.

krsharma@amity.edu

Received: 14-06-2012; Revised: 15-06-2012; Accepted: 16-06-2012.

Volume 1, Issue 2 · pp. 96–100 · PUBLISHED 10 September 2012 · DOI:

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ABSTRACT

Vigna Mungo β-amylase was immobilized onto activated Bombyx mori silk fabric by enrichment with chlorination and treated with sodium nitrate (NaNO3 ) via glutaraldehyde coupling. The immobilization of Vigna Mungo β-amylase onto activated Bombyx mori silk fabrics was excellent by having 72-84% of retention of enzyme activity. The immobilization optimum conditions such as pH, reaction time, substrate concentration, CaCl2 concentration and temperature were studied. Thermal stability of the enzyme was improved after immobilization which was 72°C as compared to free enzyme (40°C). The optimum substrate concentration and effect of CaCl2 concentration was also carried out. In addition, the immobilized enzymes had good storage stability and reusability by maintaining 60-70% of its activity after 90-120 days

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      Sharma, K. R. (2012). Immobilization of Vigna Mungo β-amylase onto NaCl and NaNO Treated Woven Bombyx Mori Silk Fabrics. Asian Journal of Biological and Life sciences, 1(2), 96–100.