Detection of Bla CTX-M gene on Resistant Salmonella enterica serovar from a Hospital in Southeast Nigeria
Adeleke, O.E.1, Onyenwe, N.E.1★, Mbata, T.I.2★ Corresponding author
- 1Department of Pharmaceutical Microbiology,University of Ibadan, NIGERIA.
- 2Department of Microbiology,Federal Polytechnic Nekede, Owerri, NIGERIA.
CORRESPONDENCE
Onyenwe, N.E.
Department of Pharmaceutical Microbiology,University of Ibadan, NIGERIA.
Received: 06-07-2012; Revised: 22-07-2012; Accepted: 03-08-2012.
Volume 1, Issue 3 · pp. 186–190 · PUBLISHED 31 December 2012 · DOI:
ABSTRACT
Typhoid fever appears to be a disease that has been associated with man since close to the first appearance of hominids. A total of twenty-five clinical isolates of Salmonella enterica serovars recovered between July-September, 2011 were investigated and screened using molecular methods for the presence of bla CTX-M type gene and plasmids. Polymerase Chain Reaction analysis showed that the four of the S.enterica encoding the CTX-M type gene CTX-M were mostly Extended Spectrum β-Lactamase producers which had no plasmids DNA. Phenotypic analysis reveals that the enzymes were active on Ceftazidime (64%) more than Cefotaxime(16%). Two folds serial dilution method reveals that Cefotaxime had increased Minimum Inhibitory Concentration (MIC) ranging from 1.56ug/ml- 12.5ug/ml depending on the isolates and the β-lactamase enzyme produced. The four blaCTX-M gene detected on the adult Patients were from the General Out Patients Department(GOPD) and National Health Insurance Scheme(NHIS) section of the hospital in Eastern Nigeria.
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O.E., A., N.E., O., & T.I., M. (2012). Detection of Bla CTX-M gene on Resistant Salmonella enterica serovar from a Hospital in Southeast Nigeria. Asian Journal of Biological and Life sciences, 1(3), 186–190.