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Asian Journal of Biological and Life sciencesNOW IN
Article

Isolation, Biochemical Characterization of Rhizobium sps SN01 Strain from Root Nodules of Mimosa pudica and their Impact on Agriculture Crops

Soundarya Shree KR1, Nagalambika Prasad1, Prakruthi G2, Siddalingeshwara KG2, Sharangouda J Patil3★★ Corresponding author

  1. 1Department of Microbiology, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka, INDIA.
  2. 2Scientific and Industrial Research Centre, Bengaluru, Karnataka, INDIA.
  3. 3Department of Zoology, NMKRV College for Women (Autonomous), Bengaluru, Karnataka, INDIA.

CORRESPONDENCE

Sharangouda J Patil

Department of Zoology, NMKRV College for Women (Autonomous), Bengaluru, Karnataka, INDIA.

shajapatil@gmail.com

Received: 05-03-2022; Revised: 27-03-2022; Accepted: 09-04-2022.

Volume 11, Issue 1 · pp. 200–205 · PUBLISHED · DOI: 10.5530/ajbls.2022.11.28

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ABSTRACT

The present study deals with the isolation of Rhizobium bacteria from root nodules of Mimosa pudica, and to check its efficiency as bio-fertilizer in improving plant growth. Rhizobium sps SN01 was isolated from root nodules of Mimosa pudica and confirmed to be Rhizobium bacteria through following biochemical tests of indole, methyl red, Voges-Proskauer and citrate utilization (IMViC), urease, hydrogen sulphide, carbohydrate fermentation tests. Rhizobium sps SN01 was analysed for antibiotic sensitivity test towards three different antibiotics such as Azithromycin, Amoxicillin and Cephalosporin. To check the efficiency, seeds of legume crops like cow peas, peas, and fenugreek were treated with Rhizobium sps, SN01, sowed in soil and coco-peat to study its germination rate and effectiveness in plant growth resulting in root length, shoot length, protein content and chlorophyll content. Impact of Rhizobium sps SN01 bacteria in soil and coco-peat quality and improvement was also focused by analyzing micro and macro nutrients responsible for its quality to improve plant growth.

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    KR, S. S., Prasad, N., G, P., KG, S., & Patil, S. J. (). Isolation, Biochemical Characterization of Rhizobium sps SN01 Strain from Root Nodules of Mimosa pudica and their Impact on Agriculture Crops. Asian Journal of Biological and Life sciences, 11(1), 200–205. https://doi.org/10.5530/ajbls.2022.11.28