jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Asian Journal of Biological and Life sciencesNOW IN
Article

In-vitro Assessment of Antibacterial and Electrochemical Properties of Methanolic Leaf Extracts of Holigarna ferruginea March. The Poisonous Plant Species in the Western Ghats

Kumbar Mudakappa Manjunath1, Yelugere Linganaik Krishnamurthy1★★ Corresponding author

  1. 1Department of PG Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Karnataka, INDIA.

CORRESPONDENCE

Yelugere Linganaik Krishnamurthy

Department of PG Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Karnataka, INDIA.

kmanjunathm1@gmail.com

Received: 14-08-2022; Revised: 18-09-2022; Accepted: 11-10-2022.

Volume 11, Issue 3 · pp. 684–689 · PUBLISHED · DOI: 10.5530/ajbls.2022.11.90

View on AJBLS original site ↗

ABSTRACT

Background:TheHoligarna ferruginea. March. (Black varnish tree) is one of the plant species belonging to the family Anacardiaceae, it is endemic to the Western Ghats. The whole genus shows potent medicinal properties in human life. on that criterion, we took actionAim: To evaluate the antibacterial properties of methanolic leaf extract of Holigarna ferruginea. March. Materials and Methods: The chopped and shade-dried leaves were extracted using Soxhlet and methanol as solvent. Preliminary phytochemical screening revealed the presence of alkaloids, phenols, flavonoids cardiac glycosides, steroids, and tannins. The current research was carried out to investigate the antibacterial activity ofHoligarna ferruginea. March. against Both Grampositive and Gram-negative bacteria, pathogens whose many species are responsible for a wide range of diseases. And also studied scavenging properties by an electrochemical method (Cyclic voltagramm).Results: The methanolic leaf extract was tested for antibacterial activity using the well diffusion technique, in which the wells with varying concentrations of the extract were prepared and used. It was at a dosage of 5mg/ml, the methanolic extracts were more effective, with greater inhibition zones in Salmonella enterica(17.66±0.57 mm), Staphylococcus aureus (17.66±0.57 mm),Escherichia coli(17.66±0.57 mm/) andKlebsiella pneumonia (16.66±0.57), then the additional concentrations of 2.5, and 1.25mg/ml were reported comparatively less zone of inhibition in all pathogenic bacteria. From this stud the Holigarna ferruginea. March. had excellent efficacy against both Gram-positive and Gram-negative bacteria. The electrochemical method was evidenced and reported moderate scavenging properties and confirms the presence of free radicals in the extract.Conclusion:These findings suggest thatH. ferruginea has antibacterial and antioxidant properties, making it a potentially useful antimicrobial phytomedicine. The reported activity backs up the plant’s traditional use in the treatment of infectious diseases.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Manjunath, K. M., & Krishnamurthy, Y. L. (). In-vitro Assessment of Antibacterial and Electrochemical Properties of Methanolic Leaf Extracts of Holigarna ferruginea March. The Poisonous Plant Species in the Western Ghats. Asian Journal of Biological and Life sciences, 11(3), 684–689. https://doi.org/10.5530/ajbls.2022.11.90