Molecular Divergence and Phylogenetic Positioning in Siluriformes Species Using mtDNA COI Gene
Mohd Imran1★★ Corresponding author
- 1Department of Zoology, Human Genetics and Toxicology Laboratory, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, INDIA.
CORRESPONDENCE
Mohd Imran
Department of Zoology, Human Genetics and Toxicology Laboratory, Section of Genetics, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, INDIA.
Received: 14-08-2023; Revised: 15-09-2023; Accepted: 22-09-2023.
Volume 12, Issue 3 · pp. 473–483 · PUBLISHED · DOI: 10.5530/ajbls.2023.12.63
ABSTRACT
Aim:Molecular divergence between species or a group of species is efficient tool to trace the relation between them and assist in their better phylogenetic positioning with good bootstrap supports. The study has emphasised on a chosen group of siluriformes species available in the nearest water resource which includes species from family Clariidae, Heteropneustidae, Bagridae, and Siluridae. Materials and Methods: Standard barcoding protocol was followed where the intraspecific and interspecific pair wise sequence divergences were calculated based on Kimura-2- Parameter model, followed by phylogenetic tree clustering. Results: A considerable gap was found in the divergence values between species which is sufficient to differentiate and delimit them. For intraspecific divergence, the average was found less than 1% whilst the interspecific divergence varied from 16 to 24%. When the comparison was made on an average basis, the divergence between species (20.40%) was found approx. 25 times higher than intraspecific divergence (0.811%). When the divergence was calculated separately at each codon position, the maximum contribution was found from 3rd codon position (78.4%) to the combined divergence which was followed by 1st codon position (6.87%) and the least contribution from 2nd codon position (1.82%). The clustering analysis with Neighbor-Joining and Maximum likelihood methods delimit the species with parallel phylogenetic clustering supporting the divergence trends. Conclusion: The work provides a thorough picture of the relationship between divergence values and how this gap in divergence values plays a critical role in differentiating species while at the same time assisting in deciphering their taxonomic and phylogenetic positioning.
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Imran, M. (). Molecular Divergence and Phylogenetic Positioning in Siluriformes Species Using mtDNA COI Gene. Asian Journal of Biological and Life sciences, 12(3), 473–483. https://doi.org/10.5530/ajbls.2023.12.63