Identification of Bioactive Compounds in Acetone Leaf and Stem-Bark Extracts of Psychotria dalzellii Hook.f. by GC-MS Analysis and Evaluation of in vitro Anti-bacterial Properties
Aralahalli Borappa Thejashree1★, Raja Naika1★ Corresponding author
- 1Department of Applied Botany, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga, Karnataka, INDIA.
CORRESPONDENCE
Aralahalli Borappa Thejashree
Department of Applied Botany, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga, Karnataka, INDIA.
Received: 14-09-2023; Revised: 21-10-2023; Accepted: 02-11-2023.
Volume 12, Issue 3 · pp. 499–509 · PUBLISHED · DOI: 10.5530/ajbls.2023.12.66
ABSTRACT
Background:Psychotria dalzelliiHook.f. (Rubiaceae) is an ethnobotanically important plant species, traditionally the stem part is used as a remedy for pruritus and the leaf juice is utilized in the preparation of eye drops for migraines. However, these claims lack of scientific validation. The current study focused on Gas Chromatography-Mass Spectrometry analysis andin vitroevaluation of antibacterial properties of leaf and stem bark acetone extracts ofP. dalzellii.Materials and Methods:P. dalzelliileaf and stem-bark samples were collected from Joida, Western Ghats, Karnataka, India. The collected plant materials were subjected to Soxhlet extraction using acetone. The presence of phytochemicals were investigated by preliminary qualitative screening and GC-MS for quantitative estimation. Then, the antibacterial potential was analysed by agar well diffusion technique against selected plant and animal pathogenic bacterial strains.Results:GC-MS chromatogram of acetone stem-bark extract shows the presence of 15 chemical constituents in that, Resorcinol (58.36%) and Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (8.81%) were found to be the major phytoconstituents. Whereas, the leaf extract shows 19 bioactive compounds in that, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (26.99%); Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (21.99%) and Resorcinol (17.87%) were found to be the major compounds.P. dalzelliiextracts of stem-bark and leaf exerted greater antibacterial activity towards all tested bacterial strains. Among leaf and stembark extracts, the stembark acetone extract were shown to be the most potent against Gram-negative pathogenic bacterial strains likePseudomonas syringae(19.33 ± 0.16 mm), Klebsiella pneumoniae (19.25 ± 0.14 mm), Xanthomonas compestris (18.16 ± 0.16 mm) and Escherichia coli (17.25 ± 0.14 mm).Conclusion:This study provides new insights into thePsychotria dalzelliias a potential tool for antibacterial drug discovery throughin vitroinvestigation that could be used to treat human and plant infectious diseases.
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Thejashree, A. B., & Naika, R. (). Identification of Bioactive Compounds in Acetone Leaf and Stem-Bark Extracts of Psychotria dalzellii Hook.f. by GC-MS Analysis and Evaluation of in vitro Anti-bacterial Properties. Asian Journal of Biological and Life sciences, 12(3), 499–509. https://doi.org/10.5530/ajbls.2023.12.66