In vitro Antioxidant and Anti-Inflammatory Activity of Dendrophthoe falcata Endophytic Fungal Ribosome Inactivating Proteins
Hemamalini Subramanyam1, Vatsala Paniyappanavara1, Monisha Chandrashekhar Patil1, Manasa Doggnal Jayappa1, Govindappa Melappa1★★ Corresponding author
- 1Department of Studies in Botany, Davangere University, Shivagangotri, Davanagere, Karnataka, INDIA
CORRESPONDENCE
Govindappa Melappa
Endophytic Fungal Natural Products Laboratory, Department of Studies in Botany, Davangere University, Shivagangotri-577007, Davanagere, Karnataka, INDIA. ; govindappa.dubot@gmail.com
Received: 19-10-2024; Revised: 22-11-2024; Accepted: 15-12-2024.
Volume 13, Issue 3 · pp. 738–747 · PUBLISHED · DOI: 10.5530/ajbls.2024.13.90
ABSTRACT
Aim: The goal of the current investigation was to characterize endophytic fungal Ribosome Inactivating Protein (RIPs) and these RIPs evaluated to analyse potentiality of antioxidant and anti-inflammatory property. Materials and Methods: Total five different endophytic fungi were subjected to identification of molecular weight by SDS-PAGE, PAS staining for glycoprotein identification and characterization of RIP by hemagglutination assay. Those RIPs evaluated for antioxidant (DPPH and FRAP) and anti-inflammatory activity (albumin denaturation study, anti-proteinase and anti-lipoxygenase assay). Results: 5 distinct types of endophytic fungus were isolated from the stem and leaves of Dendrophthoe falcata growing on Lagerstroemia speciosa. Obtained five endophytic fungal protein extracts exhibited the presence of glycoproteins with molecular weight 68 kDa. All endophytic fungal proteins are confirmed as glycoproteins in PAS staining, showed positive test for hemagglutination assay. The CcRIP and DpRIP show positive test for the erythrocytes of A, B and AB and all endophytes crude RIP also expressed the hemagglutination activity and it was depending on different blood groups. The CcRIP expressed the highest antioxidant activity in DPPH method followed by DpRIP, DpsRIP, CsRIP and DpRIP. In the FRAP assay, CcRIP and DpRIP protein extract exhibited significant antioxidant property. The CcRIP shows significant anti-inflammatory activity in all the three different methods (albumin denaturation study, anti-proteinase and anti-lipoxygenase assay). Conclusion: The study demonstrates that endophytic fungal RIPs are the potential sources of novel antioxidant and anti-inflammatory products.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Subramanyam, H., Paniyappanavara, V., Patil, M. C., Jayappa, M. D., & Melappa, G. (). In vitro Antioxidant and Anti-Inflammatory Activity of Dendrophthoe falcata Endophytic Fungal Ribosome Inactivating Proteins. Asian Journal of Biological and Life sciences, 13(3), 738–747. https://doi.org/10.5530/ajbls.2024.13.90