Cytotoxicity of Philippine Carrageenan in COS-7 Cells
Annabelle V. Briones1★, Toshinori Sato2★ Corresponding author
- 1Industrial Technology Development Institute, Department of Science and Technology DOST Complex, Gen. Santos Ave., Bicutan, Taguig City, PHILIPPINES.
- 2Deptartment of Biosciences and Informatics, Keio University 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, 223-8522, JAPAN.
CORRESPONDENCE
Annabelle V. Briones
Industrial Technology Development Institute, Department of Science and Technology DOST Complex, Gen. Santos Ave., Bicutan, Taguig City, PHILIPPINES.
Received: 15-05-2015; Revised: 09-06-2015; Accepted: 12-07-2015.
Volume 4, Issue 2 · pp. 104–108 · PUBLISHED 30 August 2015 · DOI:
ABSTRACT
This study is about the cytotoxicity of Philippine carrageenan (kappa, iota and lambda type) in COS-7 cells (African green monkey cell line producing T antigen of SV40). Results showed that among the carrageenan samples tested, lambda carrageenan has the highest cytotoxic activity as observed in the decrease of about 47.6 % cell viability when cells are incubated in longer time (48 h) with 0.0025% concentration. Shorter incubation period did not show any significant cytotoxic effect. Using a three-fold increase in concentration from 0.0025% to 0.0075%, lambda carrageenan gave the highest toxicity reading as compared to other types of carrageenan. Mild acid hydrolysis of carrageenan did not show high cytotoxic effect. Changes in cell morphology were observed at 48 hours incubation with lambda carrageenan.
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Briones, A. V., & Sato, T. (2015). Cytotoxicity of Philippine Carrageenan in COS-7 Cells. Asian Journal of Biological and Life sciences, 4(2), 104–108.