jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
Asian Journal of Biological and Life sciencesNOW IN
Article

Extraction and Molecular Characterization of Antimicrobial Metabolites from Streptomyces rochei against Bacterial Leaf Blight of Cotton Caused by Pantoea sp

Priti Patel1★, Gayatri Patel2, Priya Mehta3★ Corresponding author

  1. 1Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, INDIA.
  2. 2Department of Microbiology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, INDIA.
  3. 3Department of PGDMLT, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, INDIA.

CORRESPONDENCE

Priti Patel

Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, INDIA.

priti.patel@ ganpatuniversity.ac.in

Received: 08-05-2020; Revised: 25-06-2020; Accepted: 29-06-2020.

Volume 9, Issue 2 · pp. 158–162 · PUBLISHED 30 August 2020 · DOI: 10.5530/ajbls.2020.9.24

View on AJBLS original site ↗

ABSTRACT

India ranks among the largest producer of cotton worldwide with high economic value. During the time of cultivation cotton plant normally suffers from the bacterial blight diseases, leading to colour change of affected leaves into light brown exhibiting a blighted appearance. While retrieving the causative agent on nutrient agar plate, yellow pigmented bacteria were consistently recovered which was thought to be caused byXanthomonas oryzaepv. oryzae, the cotton bacterial blight pathogen. However, physiological and molecular analysis of isolated causative agent was of the bacteriumPantoeasp. eighteen strains of active antimicrobial metabolites producingStreptomycessp. were isolated from the soil samples of agricultural field of Mehsana district, North Gujarat, India. All the isolates were assessed for antagonistic activity againstPantoeasp. A causative agent of bacterial blight disease of cotton plant. Among the strains tested, isolate Gray-1 showed strong antimicrobial activity. This isolate was identified as aStreptomyces rocheithrough genetic analysis. The antimicrobial compounds obtained from ethyl acetate extract ofStreptomyces rocheiwere investigated using FTIR and Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS analysis showed the presence 20 bioactive compounds. The most active compounds are 3-Hydroxy-2-Methylbenzaldehyde, 2-Methyl-3-Beta-Furyl propenal, 5-Endo-5-Exo- (Epoxymethano)-6-Methylidene-7 oxabicycloheptane, 4-Hydroxy-Methylbenzaldehyde, 3Hydroxy 4 Methylbenzaldehyde and 3,4 (Methylenedioxy) Toluene, 2-Hydroxy 5 Methylisophthalaldehyde.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Patel, P., Patel, G., & Mehta, P. (2020). Extraction and Molecular Characterization of Antimicrobial Metabolites from Streptomyces rochei against Bacterial Leaf Blight of Cotton Caused by Pantoea sp. Asian Journal of Biological and Life sciences, 9(2), 158–162. https://doi.org/10.5530/ajbls.2020.9.24