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Asian Journal of Biological and Life sciencesNOW IN
Article

Effect of Free-Living Nitrogen Fixing and Phosphate Solubilizing Bacteria on Growth of Gossypium hirsutum L

Priti Patel1★, Khushbu Panchal2★ Corresponding author

  1. 1Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Gujarat, INDIA.
  2. 2Department of Microbiology, Mehsana Urban Institute of Sciences, Ganpat University, Gujarat, INDIA.

CORRESPONDENCE

Priti Patel

Department of Biotechnology, Mehsana Urban Institute of Sciences, Ganpat University, Gujarat, INDIA.

gunj_patel@yahoo.in

Received: 15-05-2020; Revised: 14-06-2020; Accepted: 21-06-2020.

Volume 9, Issue 2 · pp. 169–176 · PUBLISHED 30 August 2020 · DOI: 10.5530/ajbls.2020.9.26

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ABSTRACT

Phosphorus and nitrogen are very much crucial for growth of Plant. Nitrogen fixing bacteria and phosphate solubilizing bacteria (PSB) promote plant growth and enhance various cellular processes like root elongation, proliferation and changes of root architecture, seed development and normal crop maturity. In present study the inoculation effect of phosphate solubilizing and Nitrogen fixing bacteria, isolated from rhizosphere and non-rhizosphere soil of North Gujarat, India are investigated. The isolated bacteria were tested for indole acetic acid (IAA), ammonia production and phosphate solubilizationin vitro.Various parameters like root and shoot height, number of root hair, length, width and weight of leaves and chlorophyll content under greenhouse condition was measured. The study shows highest IAA production 0.1822 μg mL-1by isolate N8 and highest phosphate production 0.029 μg mL-1by isolate P6. The DNA of effective bacterial isolates was amplified using 16S r-DNA primers. Amplified PCR product were purified and sequenced to identify the isolates up to species level using BLAST. The isolates were identified asAzotobacter vinelandiiandBacillus niabensis, respectively (Accession number: JX564632 and KF535156).

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    Patel, P., & Panchal, K. (2020). Effect of Free-Living Nitrogen Fixing and Phosphate Solubilizing Bacteria on Growth of Gossypium hirsutum L. Asian Journal of Biological and Life sciences, 9(2), 169–176. https://doi.org/10.5530/ajbls.2020.9.26