Isolation and Identification of Thermotolerent Acetic Acid Bacteria from Waste Fruits
Bikash Kumar Sahoo1, Rashmi Ranjan Mishra1, Bikash Chandra Behera1★★ Corresponding author
- 1Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha, INDIA.
CORRESPONDENCE
Bikash Chandra Behera
Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha, INDIA.
Received: 02-06-2020; Revised: 10-07-2020; Accepted: 12-07-2020.
Volume 9, Issue 2 · pp. 209–213 · PUBLISHED 30 August 2020 · DOI: 10.5530/ajbls.2020.9.32
ABSTRACT
In the present study, thirteen thermotolerant acetic acid bacteria were isolated from waste pomegranate and apple fruit in Carr agar medium following the dilution plate technique method at 37°C. The bacterial strain capable to change the colour of the medium from green to yellow were considered asAcetobactersp. Most of the bacterial isolate could able to tolerate 3-4% of ethanol concentration, change the colour and pH of the medium and produce acetic acid in the range of 1.1-15.2 g/L. Among thirteen bacterial strains, the bacterial strain PAAB-3 could able to tolerate maximum ethanol (5%) and produce maximum acetic acid (15.2g/L) at 37°C. On the basis of morphological and biochemical characteristics the bacterial isolate, PAAB-3 is tentatively identified asAcetobacter aceti.
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Sahoo, B. K., Mishra, R. R., & Behera, B. C. (2020). Isolation and Identification of Thermotolerent Acetic Acid Bacteria from Waste Fruits. Asian Journal of Biological and Life sciences, 9(2), 209–213. https://doi.org/10.5530/ajbls.2020.9.32