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Free Radicals and AntioxidantsNOW IN
Article

In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii

Shazia Tantary1, Akbar Masood2, Aashiq Hussain Bhat1, Khalid Bashir Dar1, Mohammad Afzal Zargar1, Showkat Ahmad Ganie1★★ Corresponding author

  1. 1Department of Clinical Biochemistry, University of Kashmir, 190006, INDIA..
  2. 2Department of Biochemistry, University of Kashmir, 190006, INDIA..

CORRESPONDENCE

Showkat Ahmad Ganie

Department of Clinical Biochemistry, University of Kashmir, 190006, INDIA..

showkat_ganie786@yahoo.com

Volume 7, Issue 1 · pp. 13–22 · PUBLISHED · DOI: 10.5530/fra.2017.1.3

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ABSTRACT

Background: Euphorbia wallichii belongs to family Euphorbiaceae is used to treat various diseases on folklore levels in Kashmir valley. Objective of the study is to explore antioxidant potential and anti-inflammatory activities of Euphorbia wallichii. Materials and Methods: Antioxidant potential of extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal LPO, and hydroxyl radical scavenging activity by using standard procedures. Anti-inflammatory activity was assessed using hypotonic solution induced human erythrocyte haemolysis. Results: The highest phenolic content of 465 mg GAE/g was observed in methanol extract followed by ethyl acetate (359 mg GAE/g) and aqueous extract (291 mg GAE/g). At concentration of 700 μg/mL, DPPH radical scavenging activity of methanol extract was (94.85%) IC50 (160 μg/ml), ethyl acetate (92.68%) IC50 (200 μg/ml) and aqueous (90%) IC50 (250 μg/ml). The reducing power of the extracts increased in a concentration dependent manner. At concentration of 70 μg/mL, 92.72, 80.74 and 75.75% inhibition was observed with methanol, ethyl acetate and aqueous extract on microsomal LPO with IC50 values 31.5, 34.5 and 42 gμ/ml. Superoxide radical scavenging activity of Euphorbia wallichii extracts increased in a dose dependent manner with IC50 values 36.05 μg/ml (methanol), 45 μg/ml (ethyl acetate) and 34.5 μg/ml (aqueous extract).Euphorbia wallichii extracts exhibited antioxidant effects on Calf thymus DNA damage. At the higher concentration of plant extracts (12 μg/ml), 90, 86 and 78% increase in RBC membrane stabilization was observed with methanol, ethyl acetate and aqueous extracts of Euphorbia wallichii. Conclusion: These results clearly indicate thatEuphorbia wallichii extracts possesses the free radical savaging activity as such can be employed as potential antioxidant and anti-inflammatory agent against various oxidative stress related pathological conditions.

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    Tantary, S., Masood, A., Bhat, A. H., Dar, K. B., Zargar, M. A., & Ganie, S. A. (). In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii. Free Radicals and Antioxidants, 7(1), 13–22. https://doi.org/10.5530/fra.2017.1.3