Anti-Allergic Activity of Methanolic Extract of Lagenaria Siceraria Mol. Standley Leaves
Nidhi Jasani1, Nagja Tripathi1, Vimal Kumar2★★ Corresponding author
- 1Department of Phytopharmaceuticals and Natural Products, Institute of Pharmacy, Nirma University, Ahmedabad-382481, India.
- 2ITM School of Pharmacy, ITM Universe, Vadodara -391510, India.
CORRESPONDENCE
Vimal Kumar
ITM School of Pharmacy, ITM Universe, Vadodara -391510, India.
Volume 33, Issue 1 · pp. 35–38 · PUBLISHED 2019 · DOI:
ABSTRACT
Background: Lagenaria siceraria Mol. Standley commonly known as bottle gourd is a climbing perennial herb cultivated as a vegetable in the tropical countries. Lagenaria siceraria leaves are used traditionally for cough, bronchitis, asthma and inflammation.Purpose: On basis of ethano-medicinal uses, the anti-allergic activities of the methanolic extract of Lagenaria siceraria leaves was planned to investigate scientifically using animal models for allergic inflammation and anti-allergic activity.Method: Leaves of Lagenaria siceraria were collected from Nirma Herbal Wealth, Nirma University, Ahmedabad, Gujarat, India and the sample was identified and authenticated by ethano- botanist. The methanolic extract of the leaves was prepared from 500 gm of leaves using soxhlet extraction; it yielded 16.70 % (w/w) of dry extract. The Wistar rats were killed by exsanguinations. The pieces of mesenteries were spread and challenged with 1µg/ml of compound 48/80 for 10 min as negative control. Methanolic extract L. siceraria at different concentrations or standard ketotifen incubated mesenteries were challenged with 1µg/ml of compound 48/80 for 10 min. Pieces of mesentery were stained with 0.1% toludine blue solution and the % of degranulated mast cells were counted under microscope and the protection offered by methanolic extract / ketotifen of was calculated. In another model the Compound 48/80 (0.3μg in 0.05ml) were administered subcutaneously in the plantar region of the right hind paw of albino mice's.Result: The methanolic extract of L. siceraria (LSM) showed significant dose dependent (10-30 µg/ml) anti allergic activity against compound 48/80 induced mast cell degranulation in rat mesenteries. LSM also showed significant anti-inflammatory effects at the dose of 100 mg/kg against compound 48/80 induced paw edema in mice.Conclusion:The ethno medicinal uses of L. siceraria leaves were proved by us scientifically for treatment of allergic inflammation and anti-allergic activity using various animal models.
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Jasani, N., Tripathi, N., & Kumar, V. (2019). Anti-Allergic Activity of Methanolic Extract of Lagenaria Siceraria Mol. Standley Leaves. Indian Journal of Natural Products, 33(1), 35–38.