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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

High-performance Liquid Chromatographic Determination of Paracetamol, Propyphenazone, and Caffeine in Pharmaceutical Formulations

Kuldeep Delvadiya1, Prachi Kabra1, Ritu Kimbahune2★, Nikunj Patel1, LVG Nargund1★ Corresponding author

  1. 1Department of Quality Assurance, Nargund College of Pharmacy, BSK III stage, Bangalore - 560 085, India.
  2. 2Department of Quality Assurance, Al-Ameen College of Pharmacy, Hosur Rd, Near Lal Bagh Bangalore - 560 027, India.

CORRESPONDENCE

Ritu Kimbahune

Department of Quality Assurance, Al-Ameen College of Pharmacy, Hosur Rd, Near Lal Bagh Bangalore - 560 027, India.

rituvivekk@gmail.com

Received: 22-08-2013; Accepted: 21-01-2014.

Volume 47, Issue 4 · pp. 65–72 · PUBLISHED 2013 · DOI: 10.5530/ijper.47.4.9

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ABSTRACT

This study proposes a liquid chromatography (RPHPLC) method for quantitative analysis of the most widely prescribed combination of Paracetamol, Propyphenazone and Caffeine in tablet dosage form in presence of Rasagiline as an internal standard. The chromatography was performed on a Gracesmart C18 column (5μm, 250mm×4.6mm i.d.) using the mixture of water and 2-propanol in the ratio 80:20 v/v as mobile phase. The pH of aqueous phase was adjusted to 3.0 with 1% o-phosphoric acid and a flow rate was maintained at 1.5ml/min. The analytes were detected by measuring the absorbance at 210nm. The chromatographic analysis time for each sample was kept about 20min as Rasagiline, Paracetamol, Caffeine and Propyphenazone were eluted at 1.63, 2.36, 3.17 and 11.22 min respectively. The calibration plots were obtained between 3–90μg/ml for Paracetamol, 1.5–45μg/ml for Propyphenazone and 0.5–15μg/ml for Caffeine. The method was validated in accordance with ICH norms and method was found to be specific, accurate, precise and easy to use for routine analysis.

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    Delvadiya, K., Kabra, P., Kimbahune, R., Patel, N., & Nargund, L. (2013). High-performance Liquid Chromatographic Determination of Paracetamol, Propyphenazone, and Caffeine in Pharmaceutical Formulations. Indian Journal of Pharmaceutical Education and Research, 47(4), 65–72. https://doi.org/10.5530/ijper.47.4.9