RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India
Madhukar Garg1, Ennus Tajuddin Tamboli1, Mhaveer singh1, Karishma Chester1, Malik Zainul Abdin2, Ruby Chandna2, Sayeed Ahmad1
- 1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi -110062, India.
- 2Department of Biotechnology, Faculty of Science, Jamia Hamdard, Hamdard University, Hamdard Nagar, New Delhi -110062, India.
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Received: 25-07-2014; Revised: 14-10-2014; Accepted: 07-01-2015.
Volume 49, Issue 3 · pp. 231–239 · PUBLISHED 2015 · DOI: 10.5530/ijper.49.3.9
ABSTRACT
The present work assessed the genetic divergence amongst the accessions of vasaka collected from different sub-climatic zones of India by RAPD (Randomly Amplified Polymorphic DNA) using twenty random declaimer primers (OPA 1-OPA 20) as the plant was found to enjoyed its therapeutic efficacy in Ayurvedic and traditional system of medicines. The dendrogram constructed for cluster analysis using an un-weighted pair group method with arithmetic means (UPGMA) grouped the accessions into 2 major clusters based on win boot. Out of the twenty random primers used for studying genetic divergence sixteen primers were found to be polymorphic. Out of 20 primers 3 were found to be 100% polymorphic generating a total of 313 amplification products with an average of 19.5 products per polymorphic primer. Genetic relationships among accessions were evaluated by generating a similarity matrix based on Jaccard’s co-efficient ranging from 0.60 to 0.91. Results showed that both environmental and genetic factors were effective in observing variations. The degree of genetic variations detected among the accessions of vasaka suggested that RAPD approach seemed to be best suited for assessing with high accuracy the genetic relationships among distinct A. vasica accessions.
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Garg, M., Tamboli, E. T., singh, M., Chester, K., Abdin, M. Z., Chandna, R., & Ahmad, S. (2015). RAPD Based Assessment of Genetic Diversity of Adhatoda vasica Leaves from Different Sub- Continents of India. Indian Journal of Pharmaceutical Education and Research, 49(3), 231–239. https://doi.org/10.5530/ijper.49.3.9