Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy
Byoung-Joo Seo1, Vivek Kumar Bajpai1, Irfan Ahmad Rather1, Yong-Ha Park1★★ Corresponding author
- 1Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712- 749, KOREA.
Received: 05-01-2015; Revised: 16-03-2015; Accepted: 23-04-2015.
Volume 49, Issue 4 · pp. 282–292 · PUBLISHED 2015 · DOI: 10.5530/ijper.49.4.6
ABSTRACT
This study was aimed to partially purify the exopolysaccharide (EPS) from the culture of Lactobacillus plantarum YML009 using ethanol precipitation method with a yield of 260 mg/L. Analytical evaluationby Bradford and Phenolsulphuric methods revealed the presence of 2.2% and 68.1% total protein and total sugar contents in partially purified EPS, respectively. Further, to confirm the biological potential, the EPS was evaluated for its antioxidant activity in various scavenging models including DPPH and nitrite radicals as well as reducing power ability. The partially purified EPS (5-40 mg/mL) displayed considerable amount of antioxidant efficacy on scavenging DPPH and nitrite radicals by 44.73%, and 43.93%, respectively. Moreover, EPS showed potentreducing power capacity along with total phenolic content 18.96 μg/mg of GAE.
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Seo, B., Bajpai, V. K., Rather, I. A., & Park, Y. (2015). Partially Purified Exopolysaccharide from Lactobacillus plantarum YML009 with Total Phenolic Content, Antioxidant and Free Radical Scavenging Efficacy. Indian Journal of Pharmaceutical Education and Research, 49(4), 282–292. https://doi.org/10.5530/ijper.49.4.6