Article · Volume 50, Issue 1 · pp. 190–197
Synthesis and In vitro Cytotoxicity of a Novel Efficient Cisplatin-loaded Poly N-butyl Cyanoacrylate
Nahid Haghnazari1, Hadi Mohammadi2, Mohammad Javad Mokhtari3, Changiz Karami4, Soheil Ghasemi5
- 1Department of biology, Faculty of Science, Sanandaj Branch, Islamic Azad University, Sanandaj-Iran.
- 2Young Researchers and Elite Club, Kermanshah Branch, Islamic Azad University, Kermanshah-Iran.
- 3Young Researchers and Elite Club, Zarghan Branch, Islamic Azad University, Zarghan-Iran.
- 4Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah-Iran.
- 5Department of Pilot Nano biotechnology, Pasteur Institute of Iran, Tehran-Iran.
Abstract
Purpose: Typically, drug-loaded polymer colloidal carriers are synthesized by the drug entrapment during anionic emulsion polymerization. The purpose of this study was to trial the ability of Cisplatin-loaded Poly Butyl Cyanoacrylate (PBCA) nanoparticles.Methods: The cytotoxicity of Cisplatin-loaded PBCA nanoparticles was evaluated by MT assay. The Polymeric nanoparticles have been characterized using TEM, SEM, FTIR and DLS.Results: Polymeric nanoparticles with loaded and unloaded drug prepared by this method, have spherical structure with 350 and 230 nm in diameter and their zeta potentials were -3.2 and -10.7 mv respectively. The average amount of drug loaded on the nanoparticles was 70.88 ± 5.51%. MTT assay was carried out to evaluate the cytotoxicity property of the nanoparticles. Inhibition concentration values (IC50 ) for Cisplatin-loaded Nanoparticles were 20.8 and 18.2 μM for 24 and 48 h respectively.Conclusion: It was shown that cisplatin-loaded nanoparticles strongly increased cytotoxicity in comparison to classic drug in the MCF-7 cell line.
Keywords: Cisplatin; Drug delivery; Emulsion polymerization; Encapsulation; Polymeric Nanoparticles
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