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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms

Dobrina Doncheva Tsvetkova1, Danka Petrova Obreshkova1,2, Stefka Achkova Ivanova1,2, Bozhidarka Hadjieva3, Peter Yordanov Atanasov4

  1. 1Medical University-Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry.
  2. 2Medical University-Plovdiv, Faculty of Pharmacy, Department of Pharmacognosy and Pharmaceutical Chemistry, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
  3. 3Medical University-Plovdiv, Medical College, 15A Vasil Aprilov Str., Plovdiv 4002, BULGARIA.
  4. 4Clinic of Internal Diseases UMHATEM ”N. I. Pirogov”-SOFIA, BULGARIA.

CORRESPONDENCE

dobrinka30@abv.bg

Received: 23-04-2016; Revised: 20-06-2016; Accepted: 23-06-2016.

Volume 50, Issue 3 · pp. 482–488 · PUBLISHED 2026 · DOI: 10.5530/ijper.50.3.23

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ABSTRACT

The aim of current study was the application of validated TLC-densitometric method for identification and determination of Estradiol hemihydrate in dosage forms. The applied TLC conditions were: Silicagel G60 F254 glass plates; mobile phase: chloroform : acetone = 90 : 10 v/v, migration distance of mobile phase: 120 mm, UV-detection at  = 254 nm. All of the experimental results for the content of Estradiol hemihydrate correspond to the respective confidence interval: Estrofem table: 1.78 mg ÷ 2.12 mg; Femoston F1 table: 1.88 mg ÷ 2.2 mg; Femoston F2 table: 1.99 mg ÷ 2.19 mg; Trisequens T1 table: 1.78 mg ÷ 2.18 mg; Trisequens T2 table: 1.92 mg ÷ 2.12 mg; Trisequens T3 table: 0.97 mg ÷ 1.17 mg. The proposed validated TLC-densitometric method is appropriate for quality control of Estradiol hemihydrate in commercially available tablets.

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    Tsvetkova, D. D., Obreshkova, D. P., Ivanova, S. A., Hadjieva, B., & Atanasov, P. Y. (2026). Application of TLC-Densitometry for Analysis of Estradiol Hemihydrate in Dosage Forms. Indian Journal of Pharmaceutical Education and Research, 50(3), 482–488. https://doi.org/10.5530/ijper.50.3.23