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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF

Hai-bin Yu1, LI Li2, Zhou-xin Ren3,4★, Jun-ling Shen1★ Corresponding author

  1. 1The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000, China;.
  2. 2The Hospital of Henan Academy Institute of Traditional Chinese Medicine, Henan Zhengzhou 450000, China;.
  3. 3Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;.
  4. 4Collaborative Innovation Center for Respiratory Disease Diagnosis And Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.

CORRESPONDENCE

Zhou-xin Ren

Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China;.

renzhouxin123@126.com

Received: 03-05-2016; Revised: 30-08-2016; Accepted: 09-09-2016.

Volume 50, Issue 4 · pp. 583–590 · PUBLISHED 2016 · DOI: 10.5530/ijper.50.4.10

View on IJPER original site ↗

ABSTRACT

Transforming growth factor-β1 (TGF-β1) is a known potent inducer causing epithelial to mesenchymal transition(EMT) in some cancers. The present study was to evaluate the effect of hesperidin on EMT of non-small cell lung cancer (NSCLC) in vitro. In this study, A549 cells, a cellular line of non-small cell lung cancer, were used for evaluation of EMT induced by TGF-β1. MTT assay was used to evaluate the effect of hesperidin on cell viability with or without TGF-β1 co-culture. At the same time of TGF-β1 addition or after cellular morphological change by TGF-β1 stimulation, hesperidin was given to cells, respectively. Roundness of cells, E-cadherin, α-SMA, Col-I, MMP-9, TIMP-1 were evaluated EMT by TGF-β1 induction and effect of hesperidin on the transition. 40 and 80μM hesperidin showed significant inhibition on cellular viability without TGF-β1; co culture with TGF-β1, 40μM hesperidin showed significant inhibition on the viability but not showed significant inhibition after TGF-β1 stimulation. Whether co-culture with TGF-β1 or addition after TGF-β1, 40μM hesperidin showed inhibitory effect on epithelial to mesenchymal transition and inhibitory effect on extracellular matrix degradation of MMP-9. The above results suggest that hesperidin could be a potential candidate for inhibited migration of non-small cell lung cancer.

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    Yu, H., Li, L., Ren, Z., & Shen, J. (2016). Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF. Indian Journal of Pharmaceutical Education and Research, 50(4), 583–590. https://doi.org/10.5530/ijper.50.4.10