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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

D-Tagatose production by Lactococcus lactis NZ9000 Cells Harboring Lactobacillus plantarum L-arabinose Isomerase

Yao Zhang1, Yanli Fan1, Haijie Hu1, Haixu Yang1, Xuegang Luo1, Zhongyuan Li1, Hao Zhou1, Wenjian Ma1, Yajian Song1★, Tongcun Zhang1★ Corresponding author

  1. 1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People’s Republic of CHINA.

CORRESPONDENCE

Yajian Song

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People’s Republic of CHINA.

songyajian@tust.edu.cn

Received: 30-08-2016; Revised: 17-11-2016; Accepted: 23-11-2016.

Volume 51, Issue 2 · pp. 288–294 · PUBLISHED 2017 · DOI: 10.5530/ijper.51.2.34

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ABSTRACT

D-tagatose is a functional sweetener present in medicine, food, and dairy products and with broad market prospects, and L-arabinose isomerase gene (araA) can mediate the bioconversion of D-galactose into D-tagatose. In this study, a Lactococcus lactis NZ9000 strain harboring exogenous L-arabinose isomerase was constructed to produce D-tagatose. Lactobacillus plantarum CGMCC 8198 exhibits L-arabinose isomerase activity and its genome has been sequenced. The araA gene of Lactobacillus plantarum CGMCC 8198 encoding L-arabinose isomerase was identified by sequence analysis and was successfully cloned and expressed in Lactococcus lactis NZ9000. The D-tagatose production by the whole cell of the recombinant strain was optimized. The optimal condition for conversion reaction was at 50°C, pH 7.0 and with 300 mmol/L Mn2+ and 60 g/L galactose added. The D-tagatose yield and conversion rate at the optimal condition was determined and reached 40.2 g/L and 67%, respectively.

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    Zhang, Y., Fan, Y., Hu, H., Yang, H., Luo, X., Li, Z., Zhou, H., Ma, W., Song, Y., & Zhang, T. (2017). D-Tagatose production by Lactococcus lactis NZ9000 Cells Harboring Lactobacillus plantarum L-arabinose Isomerase. Indian Journal of Pharmaceutical Education and Research, 51(2), 288–294. https://doi.org/10.5530/ijper.51.2.34