Sub-cellular localization of Tetrodotoxin in the Tissues of the Pufferfish Takifugu Oblongus
Indumathi S M1, Samanta Sekhar Khora1★★ Corresponding author
- 1Department of Integrative Biology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil Nadu, INDIA.
CORRESPONDENCE
Samanta Sekhar Khora
Department of Integrative Biology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil Nadu, INDIA.
Received: 29-11-2016; Revised: 06-01-2017; Accepted: 15-02-2017.
Volume 51, Issue 3 · pp. 407–411 · PUBLISHED 2017 · DOI: 10.5530/ijper.51.3.68
ABSTRACT
Objective: The prime objective of this study is to determine the origin of Tetrodotoxin in the pufferfish Takifugu oblongus. Methods: Ultracentrifugation was performed to separate the tissues into various cellular fractions. Mouse bioassay was done to analyse the toxicity of different fractions from skin, liver and ovary of T. oblongus. The toxic fractions were subjected to HPLC-UV detection, to detect the presence of tetrodotoxin and quantify it. Results: Cytosolic fractions of skin, liver and ovary were extremely toxic and the presence of tetrodotoxin was proved on comparison with the retention time of standard toxin. Conclusions: These results suggest that the origin of tetrodotoxin could be endogenous or biosynthesized by the pufferfishes themselves. Clarifying the contradictions about the origins of TTX in puffers would allow researchers to accurately identify its role and toxicogenesis of the organisms which possess tetrodotoxin.
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M, I. S., & Khora, S. S. (2017). Sub-cellular localization of Tetrodotoxin in the Tissues of the Pufferfish Takifugu Oblongus. Indian Journal of Pharmaceutical Education and Research, 51(3), 407–411. https://doi.org/10.5530/ijper.51.3.68