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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Angiotensin-Converting Enzyme (ACE) Inhibitory Effects of Hazelnut Protein Hydrolysate Prepared Using Pepsin

Evren Caglar Eroglu1★, Salih Aksay2★ Corresponding author

  1. 1Alata Horticultural Research Institute, Department of Postharvest Physiology, Mersin, TURKEY.
  2. 2University of Mersin, Department of Food Engineering, Mersin, TURKEY.

CORRESPONDENCE

Evren Caglar Eroglu

Alata Horticultural Research Institute, Department of Postharvest Physiology, Mersin, TURKEY.

evcager@gmail.com

Volume 51, Issue 3S2 · pp. s417–s420 · PUBLISHED 2017 · DOI: 10.5530/ijper.51.3s.59

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ABSTRACT

Objective / Purpose: The aim of this study is to find out the effect of protein isolate and pepsin hydrolysates of hazelnut protein on Angiotensin-Converting Enzyme (ACE) inhibition. Material and Methods: Unshelled hazelnuts (Corylus avellana L.) was firstly grinded and defatted by petroleum ether. Alkaline extraction and isoelectric precipitation method was used in order to obtain protein isolate. Neutralized protein suspension was freeze dried before analysis and stored at -18ºC. Pepsin hydrolysis was conducted at pH 2 with 1:20 (w:w) enzyme/substrate ratio at 37ºC for 60 min. Hydrolysis was stopped by heating samples to 85ºC for enzyme inactivation and hydrolysates were analyzed for ACE inhibitory effect. 100 μL of 2 mM HHL, 20 μL of hydrolysate and 20 μL of 2 mU of ACE were incubated in 37ºC for 60 min. Reaction was terminated by adding 85 μl of 1 M HCl. Shimadzu HPLC system with C18 column and DAD was used for ACE inhibition analysis. Results and Discussion: Hazelnut protein isolate and peptides have over 95% ACE inhibition. On the other hand, IC50 values of samples taken at 0, 30 and 60 minutes of pepsin hydrolysis were 1.29 mg protein/ml, 0.25 mg protein/mL and 0.22 mg protein/mL, respectively. Conclusion: Results shows that hazelnut protein isolate and peptides have reasonable ACE inhibition properties. Pepsin hydrolysates of hazelnut isolates gives almost 6 times more anti-hypertensive activity than hazelnut protein isolate.

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    Eroglu, E. C., & Aksay, S. (2017). Angiotensin-Converting Enzyme (ACE) Inhibitory Effects of Hazelnut Protein Hydrolysate Prepared Using Pepsin. Indian Journal of Pharmaceutical Education and Research, 51(3S2), s417–s420. https://doi.org/10.5530/ijper.51.3s.59