Article · Volume 51, Issue 4S · pp. s783–s789
Development and Validation of Inductively Coupled Plasma Atomic Emission Spectroscopy [ICP-AES] Analytical Method for Estimation of Cisplatin in Biological Samples
Shruti Akshay Wagle1, Darshana Jain2, Sudha Rathod1, Rajani Athawale3, Amrita Bajaj4
- 1Oriental College of Pharmacy, Sector 2, Plot No 3, 4, 5, Sanpada (W), Navi Mumbai, Maharashtra, INDIA.
- 2C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (W), Mumbai, Maharashtra, INDIA.
- 3Principal K.M Kundnani College of Pharmacy, Plot No. 23, Jote Joy Building, Rambhau Salgaonkar Road, Cuffe Parade, Mumbai, Maharashtra, INDIA.
- 4SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, V.M Road, Vile Parle (W), Mumbai, Maharashtra, INDIA.
Abstract
Objective: Cisplatin, a popular anti-neoplastic agent is employed as a first line treatment for variety of cancers. Majority of the analytical methods reported for Cisplatin are either complex or not suitable for routine analysis of the drug. Hence, there is a need for development of suitable analytical technique like ICP-AES for quantitation of Cisplatin in complex matrices and biological samples.Methods: Cisplatin was analyzed in the present study based on its single step conversion to platinum metal with aqua regia.Results: The developed method was validated and was found to be linear in the range of 0.375 to 15 μg/ml with a regression coefficient of 0.992. The percent recoveries of Cisplatin from plasma and tissue samples ranged from 95 to 99%.Discussion: A simple, low cost, sensitive, one step ICP-AES technique was developed and validated for bioanalytical estimation of Cisplatin.
Keywords: Cancer; Cisplatin; ICP-AES; Platinum
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