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Indian Journal of Pharmaceutical Education and ResearchNOW IN
Article

Method Development and Validation for the Estimation of Dothiepin Hydrochloride by using RP-HPLC in PURE and Tablet Dosage Form

Prasanthi Thayi1★, Lakshman Rao Atmakuri2, Nandini Mandada3, Hemanth Mandava3, Bhuvaneswari Mandru3, Chaitanya Manne3★ Corresponding author

  1. 1Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District.
  2. 2Department of Pharmaceutical Analysis, Professor and Principal, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna.
  3. 3Student, V.V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.

CORRESPONDENCE

Prasanthi Thayi

Department of Pharmaceutical Analysis, Associate Professor, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Krishna District.

prasanthi8585@gmail.com

Received: 17-07-2018; Revised: 31-10-2018; Accepted: 18-01-2019.

Volume 53, Issue 2 · pp. 310–315 · PUBLISHED 2019 · DOI: 10.5530/ijper.53.2.39

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ABSTRACT

Aim: A simple, sensitive, accurate and precise RP-HPLC method was developed for the determination of Dothiepin HCl (DTH) in pure and tablet dosage form. Methods: The method was developed by using Phenomenex C18 (250 X 4.6 mm, 5 µm) and the mobile phase composed of buffer (0.1M sodium acetate): acetonitrile in the ratio of 50:50 v/v. The buffer pH was adjusted to 2.8. The retention time for Dothiepin HCl was found to be 3.44 min. Linearity range for Dothiepin HCl was found to be 10-60 µg/mL and the regression equation was found to be y=14691x-12844. % RSD for intra- and inter-day precision was found to be 0.27% and 0.84%. Average mean recovery was found to be 99.94%. LOD and LOQ values obtained for Dothiepin HCl were found to be 0.825 µg/mL and 2.498 µg/mL respectively. Conclusion: The results are analysed statistically and are found to be satisfactory. Hence this method can be successfully employed for analysis of Dothiepin HCl in tablet dosage form.

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    Thayi, P., Atmakuri, L. R., Mandada, N., Mandava, H., Mandru, B., & Manne, C. (2019). Method Development and Validation for the Estimation of Dothiepin Hydrochloride by using RP-HPLC in PURE and Tablet Dosage Form. Indian Journal of Pharmaceutical Education and Research, 53(2), 310–315. https://doi.org/10.5530/ijper.53.2.39